Optimized oxidoreductases for medium and large scale industrial biotransformations
Duration: 01-11-2013 to 31-10-2016
Web of the project: www.indoxproject.eu
This multidisciplinary approach is supported by a highly-specialized consortium of SMEs, large companies and research/academic institutions. The INDOX project is coordinated by Dr Angel T. Martínez from CIB-CSIC (Madrid, Spain).
The INDOX consortium brings together six highly-specialized SMEs in the areas of fungal enzymes (BIOS, Jena, Germany), computation (Anaxomics, Barcelona, Spain), biotechnological processes (Biopolis, Valencia, Spain), enzyme immobilization (CLEA, Delft, The Netherlands), industrial dyes (SETAS, Çerkezköy, Turkey), and chiral chemicals as API precursors (Chiracon, Luckenwalde, Germany); the world leading company in the sector of industrial enzymes (Novozymes, Bagsvaerd, Denmark) and a second large company of the chemical sector(Cheminova, Lemvig, Denmark) together with a selection of nine research/academic partners with high expertise in the different oxidoreductase types, namely heme-peroxidases (CIB-CSIC, ICP-CSIC, Madrid, Spain), heme-peroxygenases (TUD-IHIZ Zittau, Germany; IRNAS-CSIC, Sevilla, Spain; TUDelft, Delft, The Netherlands), flavo-oxidases (WUR, Wageningen, The Netherlands; BOKU, Vienna, Austria; others), and other copper-oxidoreductases (UNINA, Naples, Italy; INRA Marseille, France; and others).
The INDOX proposal on industrial oxidoreductases aims to provide relevant industrial case stories to demonstrate the efficacy of optimized and cost-effective oxidative biocatalysts on targeted industrial reactions, and to establish the processes scalability, sustainability and cost-efficiency versus chemical conversions.
The project flow comprises: i) Recovery of selective oxidative biocatalysts for target reactions; ii) Improvement of their oxidation activity and stability by protein engineering using rational design (based on structural-functional information), directed evolution and hybrid approaches (in yeast and other expression systems), combined with computer simulations, to fulfill the operational and catalytic conditions required by the chemical industry; iii) Optimization of reaction conditions and iV) Evaluation of cost efficiency compared to chemical processing and broad applicability for the use in the chemical industry.
ANAXOMICS participates in this project in collaboration with Barcelona Supercomputing Center (BSC) as a third party. Our role will center on the rationalization of point mutations and the prediction of new ones. For this purpose, we will use a diverse set of state of the art software, including PELE and QM/MM techniques, together with massive parallel computing. Anaxomics with this collaboration intents to advance in the molecular modeling techniques with the aim of possibly extending the services to our commercial platform.